January 16, 1965 – Jeppesen Stadium
Eastern Division Squad – Red Jersey, White Trim
| 15 | Babe Parilli | Boston | Quarterback |
| 16 | Jack Kemp | Buffalo | Quarterback |
| 22 | George Saimes | Buffalo | Defensive Back |
| 24 | Gino Cappelletti | Boston | Offensive End |
| 27 | Freddy Glick | Houston | Defensive Back |
| 34 | Cookie Gilchrist | Buffalo | Fullback |
| 36 | Sid Blanks | Houston | Halfbac |
| 38 | Don Floyd* | Houston | Defensive End |
| 40 | Dainard Paulson | New York | Defensive Back |
| 41 | Larry Garron | Boston | Fullback |
| 42 | Butch Byrd | Buffalo | Defensive Back |
| 44 | Elbert Dubenion | Buffalo | Flanker |
| 47 | Pete Jaquess | Houston | Defensive Back |
| 53 | Tom Addison | Boston | Linebacker |
| 54 | Jon Morris | Boston | Center |
| 55 | Winston Hill* | New York | Tackle |
| 58 | Mike Stratton | Buffalo | Linebacker |
| 60 | Larry Grantham | New York | Linebacker |
| 61 | Bob Talamini | Houston | Guard |
| 65 | Houston Antwine | Boston | Defensive Tackle |
| 66 | Billy Shaw | Buffalo | Guard |
| 70 | Tom Sestak | Buffalo | Defensive Tackle |
| 72 | Larry Eisenhauer | Boston | Defensive End |
| 77 | Stew Barber | Buffalo | Tackle |
| 79 | Sherman Plunkett | New York | Tackle |
| 84 | Ernie Warlick | Buffalo | End |
| 85 | Nick Buoniconti | Boston | Linebacker |
| 87 | Charlie Hennigan | Houston | Flanker |
| 89 | Bob Dee | Boston | Defensive End |
*Late number changes – Don Floyd went from 38 to 78, and Winston Hill went from 55 to 52
Western Division Squad – White Jersey, Blue Trim
| 00 | Jim Otto | Oakland | Center |
| 18 | Len Dawson | Kansas City | Quarterback |
| 19 | Lance Alworth | San Diego | Flanker |
| 20 | Billy Cannon | Oakland | Fullback |
| 21 | John Hadl | San Diego | Quarterback |
| 22 | Keith Lincoln | San Diego | Fullback |
| 23 | Goose Gonsoulin | Denver | Defensive Back |
| 24 | Willie Brown | Denver | Defensive Back |
| 28 | Abner Haynes | Kansas City | Halfback |
| 36 | Clem Daniels | Oakland | Halfback |
| 37 | Mack Lee Hill | Kansas City | Fullback |
| 43 | Bobby Hunt | Kansas City | Defensive Back |
| 45 | Dave Grayson | Kansas City | Defensive Back |
| 50 | Chuck Allen | San Diego | Linebacker |
| 55 | Frank Buncom | San Diego | Linebacker |
| 56 | Jim Fraser | Denver | Linebacker |
| 65 | Wayne Hawkins | Oakland | Guard |
| 66 | Bobby Bell | Kansas City | Linebacker |
| 72 | Jerry Sturm | Denver | Guard/Tackle |
| 73 | Jim Tyrer | Kansas City | Tackle |
| 74 | Ron Mix | San Diego | Tackle |
| 75 | Jerry Mays | Kansas City | Defensive Tackle |
| 77 | Ernie Ladd | San Diego | Defensive Tackle |
| 78 | Walt Sweeney | San Diego | Guard |
| 81 | Tommy Brooker* | Kansas City | End |
| 83 | Dave Kocourek | San Diego | End |
| 84 | Art Powell | Oakland | End |
| 85 | Buck Buchanan | Kansas City | Defensive Tackle |
| 86 | Earl Faison | San Diego | Defensive End |
*Late number change – Tommy Brooker went from 81 to 33
